Abstract
Background
MicroRNA is attracting attention as a therapeutic target for osteoarthritis. We focused
on joint capsules and synovium in lumbar facet joint osteoarthritis. The purpose of
this study was to identify microRNAs that are upregulated in lumbar facet joint capsules
and synovium with osteoarthritis.
Methods
We included patients who underwent spinal fusion for degenerative lumbar spine diseases.
We selected patients who had both early-stage and late-stage facet joint osteoarthritis
in a single individual. We extracted joint capsule and synovium samples from these
patients and isolated microRNAs. During the screening phase, we compared early-stage
and late-stage osteoarthritis samples from the same individual. We identified microRNAs
with >2-fold change in expression in 75% or more of patients with late-stage osteoarthritis
using next generation sequencing. During the technical validation phase, the same
samples were used for real-time polymerase chain reaction. We identified microRNAs
with >2-fold change in expression in 62.5% or more of patients with late-stage osteoarthritis.
Results
Of 40 patients who underwent spinal fusion, we selected eight patients with both early-stage
and late-stage facet joint osteoarthritis. During the screening phase, we identified
eight upregulated microRNAs out of 2274 microRNAs in late-stage OA. In late-stage
OA, two microRNAs (miR-133a-5p and miR-144-3p) were upregulated in seven patients
and six microRNAs (miR-133a-3p, miR-133b, miR-206, miR-20a-5p, miR-301a-3p, and miR-32-5p)
were upregulated in six patients. During the technical validation phase, we found
significant upregulation of miR-144-3p expression in late-stage osteoarthritis compared
with early-stage osteoarthritis. Expression of the other microRNAs was not significantly
different according to the paired-t test. However, miR-133a-3p, miR-133b, and miR-206
were upregulated >2-fold in 62.5% or more of patients with late-stage osteoarthritis.
Conclusions
Some of the microRNAs identified in this study might be involved in joint capsule
degeneration or synovitis.
Keywords
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Article info
Publication history
Published online: February 11, 2023
Accepted:
January 18,
2023
Received in revised form:
November 16,
2022
Received:
August 3,
2022
Publication stage
In Press Corrected ProofIdentification
Copyright
© 2023 The Japanese Orthopaedic Association. Published by Elsevier B.V. All rights reserved.
